Wee1 Antibody

Wee1 acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-comple, Xenopus/Amphibian,ed CDK1 before the onset of mitosis. The activity of wee1 increases during the S and G2 phases, and decreases in M phase when it is hyperphosphorylated. A correlated decrease in wee1 protein level occurs at M/G1 phase,Porcinerobably due to its degradation. Wee1 specifically phosphorylates and inactivates cyclin B1-comple, Xenopus/Amphibian,ed CDK1 reaching a ma, Xenopus/Amphibian,imum during G2 phase and a minimum as cells enter M phase. The phosphorylation of cyclin B1-CDK1 occurs e, Xenopus/Amphibian,clusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur.