Trypan blue, as one of cell staining dyes, has been proved to be excluded by alive cells, while could stain dead cells to blue. Through an optical microscope, the staining process can be observed and the number of cells can be quantitative counted. But whether the cells are suicide or are destroyed, this cannot be judged by this stain. Aviva's Trypan Blue Staining Cell Viability Assay Kit is developed on this working principle. Usually, the cell whose cytomembrane lost its integrity is considered as a dead cell.
After staining with trypan blue, users can record the number of cells directly through microscope, or take a photo under microscope then record the number. In this way, the accurate cell livability can be quantified.
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