TruStain fcX(TM) (anti-mouse CD16/32)

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain fcX(TM) is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.

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SPECIFICATIONS

Catalog Number

101320

Size

500 microg

Applications

FC

Hosts

Rat

Reactivities

Mouse

Clone

93

Gene Id

14130,14131

Isotype

Rat IgG2a, lambda

Clonality

Monoclonal

Dilutions

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain fcX(TM) at 1.0 microg per 106 cells in 100 microl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.

Storage Temp

4 C

Shipping Temp

Ambient RT

Storage Buffer

The CD16/32 antibody solution should be stored undiluted at 4C.

Alternative Names

Fcgamma R III/II, Ly-17

Regulatory Status

RUO