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TMB Substrate Reagent Set

TMB Substrate Reagent Set

Cat no: 421101

Supplier: BioLegend
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The TMB Substrate Reagent Set contains 100 ml Substrate Reagent A and 100 ml Substrate Reagent B, sufficient for twenty 96-well plates. It is formulated with 3, 3', 5, 5' tetramethyl benzidine (TMB). The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H2SO4 or 1M H3PO4 (resulting in a yellow color), and read at 450 nm.
Catalogue number: 421101
Applications: ELISA
Size: 100 ml
Dilutions: Substrate Reagent A should be clear and colorless. Substrate Reagent B should be colorless to a very light amber.TMB Substrate is the mixture of equal volumes of TMB Substrate Reagent A with TMB Substrate Reagent B. For example, for one plate, mix 6 ml Substrate Reagent A with 6 ml of Substrate Reagent B in a clean container. Mix immediately before use. After mixing the Reagents together, TMB substrate working solution should be colorless or very faint blue.
Storage buffer: The TMB Reagent Set (Substrate Reagent A and Substrate Reagent B) should be stored at 4C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: TMB Substrate
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