This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to Tau protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.