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Stop Solution for TMB Substrate
BioLegend

Supplier:

BioLegend

Cat no: 423001

Stop Solution for TMB Substrate

Biolegend’s Stop Solution for TMB Substrate is specifically formulated to terminate the HRP/TMB reaction and stabilizing the color development for ELISA applications. TMB is a chromogenic substrate used in ELISA. TMB can act as a proton donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as HRP. The resulting diimine causes the solution to take on a blue color, and this color change can be read at 650 nm. The reaction can be stopped by addition of stop reagent, and the color turns from blue to yellow with 2-3 fold increased absorbance values at 450 nm.

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SPECIFICATIONS

Catalog Number

423001

Size

60 ml

Applications

ELISA

Storage Temp

Ambient RT

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Store at room temperature.

Regulatory Status

RUO

SUPPLIER INFO

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

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