This antibody reacts with human Sprouty-2 protein and is useful for determining its presence in ELISA, immunoblotting and immunoprecipitation. Approximately 20 ?l of antibody is used to immunoprecipitate hSpry2 protein from 1 mg of a HeLa cell lysate over expressing Sprouty-2 in 1 ml. Incubate the antibody for 2 h with the cell lysate. Immunoprecipitate the complex with Protein G agarose. Reactivity in other immunoassays has not yet been fully determined. A 38 kDa band corresponding to human Sprouty-2 is observed.