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SMAD3 phospho S423/phospho S425 Antibody

SMAD3 phospho S423/phospho S425 Antibody

Cat no: 600-401-919S


Supplier: Rockland Immunochemicals, Inc.
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This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
Catalogue number: 600-401-919S
Reactivities: Human, Mouse, Rat, Bovine, Chicken/Bird, Porcine
Hosts: Rabbit
Size: 25
Unit: uL
Storage buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration: 1.0 mg/mL
Format: IgG
Clonality: Polyclonal