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RFP Antibody

Cat no: 600-901-379S

RFP Antibody

Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

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SPECIFICATIONS

Catalog Number

600-901-379S

Size

25

Unit

uL

Format

IgG

Clonality

Polyclonal

Concentration

1.0 mg/mL

Storage Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

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