Recombinant Mouse IL-21 (carrier-free)

IL-21 was identified by a functional screening from conditioned medium of activated T cells using Baf3-IL-21R transfectans. In normal B cells, IL-21 can mediate cell proliferation, growth arrest, and terminal differentiation, or apoptosis, depending on their activation status. IL-21 enhances B cell proliferation following incubation with an activating CD40 antibody. This result suggests that IL-21 enhances B cell function following T:B cell interactions. Nevertheless, IL-21 inhibits the proliferation of murine and human B cells stimulated with anti-IgM antibodies. In addition, IL-21 enhaces proliferation, cytotoxic activity, and IFNgamma production by CD8-effector T cells, and induces terminal differentiation of activated natural killer (NK) cells. Also, IL-21 can drive Th17 responses in conjunction with TGFbeta. Nevertheless, IL-21 is not essential for the differentiation of Th17 cells in vitro and in vivo, as it was showed using IL-21 and IL-21R-deficient mice. IL-6 induces the production of IL-21 from CD4 T cells upon TCR stimulation, and IL-6 and IL-21 are key players in the Tfh differentiation, characterized by increased protein expression of both Bcl6 and CXCR5. IL-21R complex is formed with the IL-21R-alpha chain and the common subunits (gammac) shared with other interleukins such as IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21R is wildly expressed on T cells, B cells, NK cells, and dendritic cells in all lymphoid tissues, as well as some nonimmune cells, such as fibroblasts and epithelial cells.

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SPECIFICATIONS

Catalog Number

574506

Size

100 microg

Gene Id

60505

Storage Temp

-20 C or -70 C

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4C for one month or from -20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

Interleukin-21

Regulatory Status

RUO