Recombinant Mouse CCL8 (MCP-2) (carrier-free)

CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells stimulated with IL-1beta. CCL8 (MCP-2) has structural and functional similarity with MCP-1 and MCP-3, and it is often co-induced with those chemokines in mononuclear cells. IFNgamma is a strong inducer of CCL8 in monocytes and fibroblasts. CCL8 can be processed and secreted as a truncated chemokine CCL8 (6-75). The truncated isoform does not chemoattract monocytes, but it binds to the CCR2 receptor and induces its internalization; as a result, CCL8 inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of Th2 cell homing, which drives IL-5-mediated chronic allergic inflammation. In addition, CCL8 binds CCR5 receptor with high affinity and inhibits the replication of human immunodeficiency virus type 1.CCL8 gene expression (by splenic stromal cells) is potentially relevant to the regulation of dendritic cell (DC) development. In addition, CCL8 expression is induced in splenic stromal cells during L. donovani infection and, in conjuction with CXCL12, hematopoietic stem and progenitor cells (HSPCs) are chemoattracted and potentially differentiated into regulatory DCs. Finally, CCL8 is a potential biomarker for the diagnosis of graft-versus-host diseases, tuberculosis, and sepsis.

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SPECIFICATIONS

Catalog Number

581704

Size

25 microg

Gene Id

20307

Storage Temp

-20 C or -70 C

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in buffer (PBS, DPBS, HBSS, or EBSS) containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution the cytokine can be stored at 4C for one month or from 20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

Monocyte chemotactic protein2, MCP-2; Small inducible cytokine subfamily A, member 8, SCYA8

Regulatory Status

RUO