Recombinant Mouse CCL2 (MCP-1) (carrier-free)

CCL2, commonly known as MCP-1, is a member of the CC beta chemokine family. It is widely expressed in endothelial cells, smooth muscle cells, and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1beta (IL-1beta), and oxidized low density lipoprotein. Several recent in vivo studies have disclosed critical roles of CCL2 in atherosclerosis. In addition, CCL2 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. CCL2 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervix, ovary, sarcoma, and prostate. In addition, CCL2 plays a key role in the regulation of MMPs during transmigration. CCL2 induces migration of MonoMac6 by induction of MMP2. CCL2 and CCL3/MIP-1alpha mediate firm adherence and (subsequent) transmigration of neutrophils via protein synthesis and secondary generation of leukotrienes and PAF, which in turn directly activate neutrophils in mouse cremaster muscle. CCL2 is induced from endothelial cells by MIF and from fetal skin fibroblasts by IL-17.

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SPECIFICATIONS

Catalog Number

578406

Size

100 microg

Gene Id

20296

Storage Temp

-20 C or -70 C

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in sterile buffer (PBS, DPBS, HBSS, or EBSS) containing carrier protein such as 1% BSA or HSA. For long-term storage, aliquot into polypropylene vials and store in a manual defrost freezer. After dilution, the cytokine can be stored at 4C for one month or from -20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

Monocyte chemoattractant protein-1, MCAF, JE, SCYA2, HC-11, P6, SMC-CF, CCL2

Regulatory Status

RUO