Recombinant Mouse CCL12 (MCP-5) (carrier-free)

Mouse CCL12 belongs to the CC chemokine family and is most closely related to human CCL2 (MCP-1) in structure (66% amino acid identity). CCL12 binds to cell surface receptor CCR2, a human CCL2 primary receptor, and can induce migration and Ca2+ flux. CCL12 is a potent chemoattractant for peripheral blood monocytes but only has weak activity on eosinophils at high doses. CCL12 is produced by macrophages during early allergic reactions and infections. During murine MCV infection, bone marrow F4/80 (+) cells can produce high levels of CCL12. The expression of CCL12 can also be upregulated by bacterial infections including pneumococcal meningitis and polymicrobial septic peritonitis. LPS can induce the expression of CCL12 in liver macrophages (Kupffer cells) in vivo. CCL12 is involved in the pathogenesis of pulmonary fibrosis by recruiting fibrocytes to the injury site and enhancing fibrotic responses. Also, CCL12 is implicated in liver and kidney fibrosis. It has also been shown that hypoxia inducible factor 1 is involved in hypoxia-induced upregulation of CCL12 in astrocytes. In addition, CCL12 is involved in joint and growth plate development.

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SPECIFICATIONS

Catalog Number

587904

Size

25 microg

Gene Id

20293

Storage Temp

-20 C or -70 C

Concentration

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored at 4C for one month or from -20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

C-C motif chemokine 12, Monocyte Chemoattractant Protein 5, MCP-1-related chemokine, Small-inducible cytokine A12, MCP5

Regulatory Status

RUO