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Recombinant Human TNF-alpha (ELISA Std.)

Recombinant Human TNF-alpha (ELISA Std.)

Cat no: 570109

Supplier: BioLegend
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TNF-alpha is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-alpha is released from cells by proteolysis of the integral membrane precursor form of TNF-alpha. TNF-alpha binding to some TNF-alpha receptors induces apoptosis and depending on cell type, receptor expression, and signal transduction status can induce other responses. TNF-alpha is involved in the inflammatory response.
Catalogue number: 570109
Applications: ELISA
Size: 4 pack
Gene: 7124
Dilutions: Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store unopened vials at 4C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Tumor necrosis factor-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
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