Recombinant Human TNF-alpha (ELISA Std.)

TNF-alpha is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-alpha is released from cells by proteolysis of the integral membrane precursor form of TNF-alpha. TNF-alpha binding to some TNF-alpha receptors induces apoptosis and depending on cell type, receptor expression, and signal transduction status can induce other responses. TNF-alpha is involved in the inflammatory response.

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SPECIFICATIONS

Catalog Number

570109

Size

4 pack

Applications

ELISA

Gene Id

7124

Dilutions

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store unopened vials at 4C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Alternative Names

Tumor necrosis factor-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2

Regulatory Status

RUO