Recombinant Human TGF-beta3 (carrier-free)

Human TGF-beta3 precursor consists of two disulfide-linked identical subunits with 412 amino acids each. The carboxy-terminal (112 residues) of TGF-beta3 represents the biologically active form of this protein; this portion of TGF-beta3 exhibits 86/112 and 89/112 identical residues compared with TGF-beta1 and TGF-beta2, respectively. TGF-beta3 precursor is cleaved by furin, and localized in the residue 300 of the trans-Golgi network. Furin processes the TGF-beta3 precursor at the carboxyl side of the consensus sequence RKKR which precedes the NH2-terminal Ala 301 residue of the mature TGF-beta3. The TGF-beta3 precursor includes (in the N-terminal portion) the latency-associated peptide (LAP dimer) and (in the C-terminal) the 25 kD portion that constitutes the mature TGF-beta3. The LAP dimer and the TGF-beta3 mature protein remain non-covalently associated after furin cleavage, and this complex does not bind to the TFG-beta receptor. In addition, the TGF-beta3 latent complex is joined covalently through LAP to LTBP. The TGF-beta1 active form requires dissociation from LAP. Some activators can release TGF-beta1 from LAP, such as thrombospondin-1, reactive oxygen species, and the integrins avb6 and avb8, and these activators might release TGF-beta3 through similar mechanism. TGF-beta3 induces biomechanical remodeling in avian embryonic atrioventricular valves. Also, gene delivery of a mutant TGF-beta3 reduces re-epithelialization density and fibroblast/myofibroblast transdifferentiation within the wound area, both mechanisms are indicative of reduced scar tissue formation. In fact, recombinant TGF-beta3 has been used in clinical trials as prophylactic treatment of human scars.

Prices direct from BioLegend

Quick response times

Exclusive Absave savings/discounts

SPECIFICATIONS

Catalog Number

585802

Size

5 microg

Gene Id

7043

Storage Temp

-20 C or -70 C

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HAS or 10 % FBS. After dilution, the cytokine can be stored at 4C for one month or from -20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

Transforming growth factor beta 3 (TGF b3), TGFB3, TGF beta 3,ARVD

Regulatory Status

RUO