Recombinant Human TGF-beta2 (carrier-free)

Human TGF-beta2 consists of two disulfide-linked, identical subunits, and displays 71.4% sequence homology with TGF-beta1. TGF-beta2 is synthesized in cells as a 442 amino acid. Furin cleaves the protein, yielding an N-terminal cleavage product which corresponds to latency-associated peptide (LAP), and the 25 kD C-terminal portion of the precursor constitutes the mature TGF-beta2. TGF-beta activators can release TGF-beta from LAP. These activators include proteases that degrade LAP, thrombospondin-1, reactive oxygen species, and integrins avb6 and avb8. TGF-beta2 is the predominant form of TGF-beta in ocular tissues, and elevated levels of TGF-beta2 have been found in the aqueous humor of patients with primary open-angle glaucoma (POAG), a major cause of blindness worldwide. There is an accumulation of extracellular matrix (ECM) in the trabecular meshwork (TM) of glaucoma patients, and TGF-beta2 seems to be responsible for this ECM increase. It has been identified that bone morphogenetic protein-4 and 7 (BMP4, BMP7) are potent antagonists of the fibrogenic effects of TGF-beta2 on human TM cells. Smad7 seems to participate in the antagonistic effect of BMP7 on TGF-beta2 signaling.

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SPECIFICATIONS

Catalog Number

583301

Size

5 microg

Gene Id

7042

Storage Temp

-20 C or -70 C

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HAS or 10 % FBS. After dilution, the cytokine can be stored at 4C for one month or from -20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

Transforming growth factor beta 2 (TGFb2, TGF beta 2),Glioblastoma-derived T-cell suppressor factor (G-TSF, GTSF)

Regulatory Status

RUO