Recombinant human IL-1beta (ELISA Std.)

IL-1beta in humans and mice does not encode a typical signal peptide and, as a result, newly synthesized pro-IL-1beta accumulates within the cytoplasm of activated monocytes and macrophages (1). Conversion of the inactive pro-IL-1beta to its mature form requires the proteolytic action of IL-1beta-converting enzyme (ICE), also termed caspase-1 (2). Secretion of mature IL-1beta from LPS-activated monocytes/macrophages is not a constitutive process. These cells must encounter a secondary stimulus that specifically activates the posttranslational processing events (2). Moreover, owing to its pro-inflammatory nature, IL-1beta is regarded as a tumor-promoting cytokine. In fact, enhanced tumor metastasis and angiogenesis has been observed under the influence of IL-1beta (3). IL-1beta is able to facilitate tumor progression in murine models of lung cancer. In addition, upregulation of metastasis and tumor angiogenesis by IL-1beta has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor (4).

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SPECIFICATIONS

Catalog Number

579409

Size

4 pack

Applications

ELISA

Gene Id

3553

Dilutions

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/ml to 2 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store unopened vials at 4C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Alternative Names

Interleukin-1beta, Catabolin, H1, IFNbeta-inducing factor, OAF

Regulatory Status

RUO