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Recombinant Human IL-12 (p70) (ELISA Std.)

Recombinant Human IL-12 (p70) (ELISA Std.)

Cat no: 573009

Supplier: BioLegend
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Bioactive IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells, and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12. Recombinant human IL-12 is a 70 kD protein containing a 306 amino acid p40 subunit and a 197 amino acid p35 subunit.
Catalogue number: 573009
Applications: ELISA
Size: 4 pack
Gene: 3592,3593
Dilutions: Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 1000 pg/ml to 15.6 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store unopened vials at 4C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Interleukin-12 p70, p40 and p35 cystine linked subunits, CLMF, NKSF, TcMF, TSF
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