Recombinant Human CCL15 (MIP-1delta) (carrier-free)

CCL15 was discovered by analyzing the upstream region of the CCL14 gene. CCL15 is arranged in tandem with CCL14 on human chromosome 17. CCL15, CCL6, CCL9, and CCL23 have an extended N-terminal domain upstream of the first two conserved chemokine cysteines. This domain is encoded by a different exon and includes 16 to 20 residues. CCL15 (1-92) is processed by cathepsin G to produce highly active forms of CCL15 [(24-92), (27-92) and (29-92)], and elastase processes CCL15 (1-92) predominantly to CCL15 (22-92). Also, CCL15 is cleaved by MT6-MMP/MMP25 to produce CCL15 with very high activity. MMP25 is localized primarily in neutrophil gelatinase granules, and it is released by neutrophil activation and degranulation in inflammatory reactions. High levels of CCL15 were detected in human renal cell carcinoma bone metastasis, and in vitro studies showed that CCL15 stimulates chemotaxis of osteoclast progenitors and enhances their differentiation in response to RANKL. CCL15 is abundant in bronchoalveolar lavage of patients with pulmonary sarcoidosis. Also, it is elevated in the serum of patients with severe asthma, and it is reduced by omalizumab, a humanized anti-IgE antibody that has been use in the treatment of asthma. It has been suggested that airway smooth muscle cells and basophils produce CCL15, and it might have an important role in asthma severity and persistence. In addition, high levels of CCL15 have been detected in the serum of patients with hepatocellular carcinoma, and human hepatic carcinoma cells express CCR1 and CCL15.

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SPECIFICATIONS

Catalog Number

587408

Size

500 microg

Gene Id

6359

Storage Temp

-20 C or -70 C

Concentration

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored at 4C for one month or from -20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

Macrophague inflammatory protein 1delta (MIP1delta), Macrophague inflammatory protein 5 (MIP5), Leukotactin 1 (LKN1), Hemofiltrate chemokine 2 (HCC-2), New CC chemokine 3 (NCC3), HCC2, MIP1 delta, MIP1d

Regulatory Status

RUO