Recombinant Human CCL14 (HCC-1) (carrier-free)

CCL14 is a homeostatic chemokine present at high concentrations in normal human plasma. CCL14 shares 46% sequence identity with CCL3 and CCL4, and it is a weak CCR1 agonist. The NH2- terminus of CCL14 can be cleaved by urokinase plasminogen activator and/or plasmin, and the resulting CCL14 (9-74) chemokine becomes a strong agonist for CCR1, CCR5, and a weak agonist for CCR3. Subsequently, CCL14 (9-74) is a substrate for dipeptidyl peptidase (CD26) that removes two amino acids to produce CCL14 (11-74), which is inactive. CCL15 and CCL23 similarly have an extended N-terminal domain that is cleaved, which activates them. CCL14, in conjuction with CX3CL1 and CCL4, promotes trophoblast migration at the feto-maternal interface. In addition, it has been published that modifying CCL14, eliminating amino acids 1-8 and substituting amino acid in position 9 by nonanoic acid, led to the identification of a potent CCR3 agonist, n-nonanoyl (NNY)-CCL14 (10-74). NNY-CCL14 induces release of reactive oxygen species and leads to chemotaxis of human eosinophils. Also, it acts as an agonistic inactivator of CCR1, CCR2, CCR3, and CCR5. CCL14 is constitutively expressed in different tissues, and its concentration in plasma increases up to 10-fold in inflammatory conditions and in chronic renal failure.

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SPECIFICATIONS

Catalog Number

587206

Size

100 microg

Gene Id

6358

Storage Temp

-20 C or -70 C

Concentration

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored at 4C for one month or from -20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

Hemofiltrate chemokine 1 (HCC1), New CC chemokine 2 (NCC2), Small inducible cytokine subfamily A, member 14 (SCYA14)

Regulatory Status

RUO