RBC Lysis/Fixation Solution (10X)

Size

50 ml

Dilutions

Whole Blood Lyse/Wash Procedure:1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts of room temperature dI H20. 2. Mix 100 microl of whole blood with fluorochrome-conjugated antibody. 3. Incubate at room temperature, in the dark, for 20 minutes. 4. Add 2.0 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately. 5. Incubate at room temperature, in the dark, for 15 minutes. 6. Centrifuge at 350Xg for five minutes. Discard supernatant. 7. Add 2 mL BioLegend Cell Staining Buffer (Cat No 420201). 8. Centrifuge at 350Xg for five minutes. Discard supernatant. 9. Resuspend cell pellet in 0.3-0.5 mL Cell Staining Buffer. 10. Analyze cells immediately by flow cytometry or store at 4C, in the dark, for no longer than 12 hours. Whole Blood Lyse/No Wash Procedure:1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts room temperature dI H20. 2. Mix 50 microl of whole blood with fluorochrome-conjugated antibody. 3. Incubate at room temperature, in the dark, for 20 minutes. 4. Add 0.45 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately. 5. Incubate at room temperature, in the dark, for 15 minutes. 6. Analyze samples by flow cytometry.

Concentration

Storage Buffer

Store RBC lysis buffer at 4C.

Regulatory Status

RUO