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Products RBC Lysis/Fixation Solution (10X)
| Catalogue number: | 422401 |
| Applications: | Flow Cytometry |
| Size: | 50 ml |
| Dilutions: | Whole Blood Lyse/Wash Procedure:1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts of room temperature dI H20. 2. Mix 100 microl of whole blood with fluorochrome-conjugated antibody. 3. Incubate at room temperature, in the dark, for 20 minutes. 4. Add 2.0 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately. 5. Incubate at room temperature, in the dark, for 15 minutes. 6. Centrifuge at 350Xg for five minutes. Discard supernatant. 7. Add 2 mL BioLegend Cell Staining Buffer (Cat No 420201). 8. Centrifuge at 350Xg for five minutes. Discard supernatant. 9. Resuspend cell pellet in 0.3-0.5 mL Cell Staining Buffer. 10. Analyze cells immediately by flow cytometry or store at 4C, in the dark, for no longer than 12 hours. Whole Blood Lyse/No Wash Procedure:1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts room temperature dI H20. 2. Mix 50 microl of whole blood with fluorochrome-conjugated antibody. 3. Incubate at room temperature, in the dark, for 20 minutes. 4. Add 0.45 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately. 5. Incubate at room temperature, in the dark, for 15 minutes. 6. Analyze samples by flow cytometry. |
| Storage buffer: | Store RBC lysis buffer at 4C. |
| Regulatory status: | RUO |
| Shipping temp: | Ambient RT |
| Storage temp: | 4 C |
| Alternative names: | Fix/Lyse, FACS Lysing Solution |
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