Rat Anti- Mouse CD25/IL- 2Ra- SPRD(0.1 mg)

The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.¹⁴ CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.^{2, 3, 15} Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.⁴ CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.⁵ It is expressed at a higher level on CD4⁺CD8⁺ thymocytes.^{5, 6} It is also expressed on cultured epidermal Langerhans cells.⁷ The biochemical features of murine CD25 have been characterized in detail.^8-10 The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4.^{2, 11, 12} 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation d