Purified anti-TPX2

The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100 kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, alpha-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738. The 18D5 monoclonal antibody recognizes human TPX2 and has been shown to be useful for Western blotting.

Prices direct from BioLegend

Quick response times

Exclusive Absave savings/discounts

SPECIFICATIONS

Catalog Number

628001

Size

25 microg

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Clone

18D5

Antigen

Amino Acid: 1-220 of human TPX2

Gene Id

22974

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Targeting protein for Xklp1

Regulatory Status

RUO