Purified anti-TAF250

TAF250 (also known as TAF1 RNA polymerase II, TATA box binding protein associated factor 250 kD, UBF initiation factor TFIID 250 kD subunit) is a 210-250 kD member of the bipartite protein kinase family containing protein kinase domains. There are two reported isoforms of this ubiquitously expressed nuclear protein. Highest expression of TAF250 has been observed in thymus, glioblastoma, and normal endometrium at mid-secretory phase. TAF250 functions as acetyltransferase and ubiquitin activating/conjugating enzyme. This protein is thought to play an essential role in TFIID assembly by interacting with TBP and other TAFs, linking cell cycle and transcriptional control. TAF250 is essential for the progression of G1 phase of the cell cycle and has been shown to stimulate ribosomal DNA transcription by binding to the activator UBF. TAF250 is modified by phosphorylation (casein kinase II). TAF250 is the largest subunit of TFIID and has been shown to interact with TBP and other TAF proteins, and UBF. The Poly6105 antibody recognizes both isoforms of human TAF250 and has been shown to be useful for Western blotting.

Prices direct from BioLegend

Quick response times

Exclusive Absave savings/discounts

SPECIFICATIONS

Catalog Number

610502

Size

200 microl

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6105

Antigen

Peptide

Gene Id

40813

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

TAF1 RNA polymerase II, TATA box binding protein associated factor 250 kD, UBF initiation factor TFIID 250 kD subunit

Regulatory Status

RUO