Purified anti-STAT1

STAT1, also known as signal transduction and activator of transcription-1, is a ubiquitously expressed cytoplasmic protein that becomes phosphorylated in response to cytokine signaling (interferon alpha, interferon gamma, EGF, PDGF, and IL-6) by receptor-associated kinases and translocates to the nucleus to act as a transcriptional activator. Two isoforms of STAT1 exist as a result of alternative RNA processing with apparent molecular weights of 88 and 91 kD. STAT1 has been shown to interact with a number of proteins including STAT3, FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27 receptor, IL-2R beta and gamma, IFNalphaRbeta, STAT2, c-Src, and many others. STAT1 knockout mice are highly sensitive to infection with microbial infections and viruses. The SAT-84 monoclonal antibody recognizes human STAT1 and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

627302

Size

100 microg

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Clone

SAT-84

Antigen

STAT1 peptide QLDSKFLEQVHQLYD

Gene Id

6772

Isotype

Mouse IgG1

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2-4 microg/ml working concentration of antibody in 5% BSA for each mini-gel. For IHC, use a 15 microg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

Signal transduction and activator of transcription-1, STAT-1

Regulatory Status

RUO