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Purified anti-Spt16 (FACT140 complex)

Purified anti-Spt16 (FACT140 complex)

Cat no: 607002

Supplier: BioLegend
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Spt16 is a 120-140 kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. The 8D2 monoclonal antibody recognizes human Spt16 and has been shown to be useful for Western blotting.
Catalogue number: 607002
Reactivities: Human
Hosts: Mouse
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100 microg
Clone: 8D2
Gene: 852665
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store at 4C.
Concentration: 0.5 mg/ml
Antigen: Recombinant (full length)
Isotype: Mouse IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Facilitates Chromatin Transcription (FACT140), Spt 16 protein, Cdc68
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