Purified anti-Spt16 (FACT140 complex)

Spt16 is a 120-140 kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. The 8D2 monoclonal antibody recognizes human Spt16 and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

607001

Size

25 microg

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Hum

Clone

8D2

Antigen

Recombinant (full length)

Gene Id

852665

Isotype

Mouse IgG2a, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store at 4C.

Alternative Names

Facilitates Chromatin Transcription (FACT140), Spt 16 protein, Cdc68

Regulatory Status

RUO