Purified anti-SIRT1

SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors. The Poly6343 antibody has been shown to be useful for western blotting and immunoprecipitation of human SIRT1 protein.

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SPECIFICATIONS

Catalog Number

634301

Size

50 microl

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6343

Antigen

Peptide mapping to the carboxy terminal domain of human SIRT1.

Gene Id

23411

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use10microl per 5 ml antibody dilution buffer for each mini-gel.It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. DO NOT FREEZE..

Alternative Names

NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1, hSIRT1, hSIR2

Regulatory Status

RUO