Purified anti-RUNX3

RUNX3 belongs to the runt-domain family of transcription factors that regulates gene expression in major developmental pathways, such as T-cell differentiation and neurogenesis. RUNX3 is epigenetically silenced in many types of cancers. In gastric cancer, RUNX3 has been frequently reported to be inactivated by gene silencing or protein mislocalization. The oncoprotein SRC phosphorylates RUNX3 at multiple tyrosine residues, resulting in cytoplasmic mislocalization of RUNX3. RUNX3 is regulated by the TGF-B/Smad pathway, and induces apoptosis or cell cycle arrest of gastric cancer cells through upregulating expression of p21 and Bim. The loss of RUNX3 function in T-cells cause suppression of Treg function and formation of colitis tumor. These findings suggest an important role of RUNX3 in tumor suppression.

Prices direct from BioLegend

Quick response times

Exclusive Absave savings/discounts

SPECIFICATIONS

Catalog Number

653601

Size

25 microg

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum

Clone

9F4A17

Antigen

Partial human RUNX3 recombinant protein (194-304 aa).

Gene Id

864

Isotype

Mouse IgG2a, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1-0.5 microg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

Runt-related transcription factor 3, Polyomavirus enhancer-binding protein 2 alpha C subunit (PEBP2 alpha C), Acute myeloid leukemia 2 (AML2)

Regulatory Status

RUO