Purified anti-rat IFN-gamma

Interferon-gamma is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-gamma also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-gamma can upregulate MHC class I and II antigen expression by antigen-presenting cells. The DB-1 antibody reacts with rat and mouse interferon-gamma (IFN-gamma). The DB-1 antibody can neutralize the bioactivity of natural or recombinant IFN-gamma. The DB-1 antibody has been well characterized for ELISPOT, ELISA, intracellular staining, Western blotting, IHC, and neutralization (in vitro and in vivo).

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SPECIFICATIONS

Catalog Number

507801

Size

50 microg

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Clone

DB-1

Antigen

Recombinant rat IFN-gamma

Gene Id

25712

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA analysis. For ELISA capture applications, the suggested use of this reagent is 1-4 ug/ml. To obtain a linear standard curve, serial dilutions of IFN-gamma recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. The purified DB-1 has been tested by blocking fluorochrome conjugated DB-1 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (? 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Interferon-gamma, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Regulatory Status

RUO