Purified anti-PRC1

PRC1 (also known as protein regulator of cytokinesis 1 and mitotic spindle-associated CDK substrate) is a member of the MAP65/ASE1 family with a molecular weight of approximately 72 kD. PRC1 localization changes with cell-cycle position and can be found in the nucleus, the mitotic spindle, and the cell mid-body. PRC1 is a mitotic spindle-associated CDK substrate protein required for cytokinesis and maintenance of the spindle midzone. PRC1 can be modified by phosphorylation and has been shown to interact with Cdc2 and Cdk2. The 6G2 antibody recognizes the human PRC1 protein and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

629002

Size

100 microg

Applications

WB

Hosts

Mouse

Reactivities

Hum

Clone

6G2

Antigen

Amino Acid: 1-150

Gene Id

9055

Isotype

Mouse IgG2b, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Protein regulator of cytokinesis 1, Mitotic spindle-associated CDK substrate, Protein regulating cytokinesis 1

Regulatory Status

RUO