Purified anti-Paxillin

Paxillin (also known as peroxisomal membrane protein 3) is a cytoplasmic protein with LIM metal-binding repeat homology and LIM domains. There are three isoforms of this protein designated as alpha, beta, gamma; approximate molecular weight 68 kD. Paxillin is localized to actin-membrane attachment at sites of cell adhesion to extracellular matrix (focal adhesion). This protein undergoes cell adhesion-dependent tyrosine phosphorylation. Paxillin has been shown to interact with FAK, vinculin, src, Crk, p210BRC / ABL, talin, and integrin-beta1. The PAX227 monoclonal antibody recognizes human and mouse paxillin and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

624001

Size

50 microl

Applications

IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Clone

PAX227

Antigen

Recombinant full length

Gene Id

5829

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Peroxisomal membrane protein 3

Regulatory Status

RUO