Purified anti-p53

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.

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SPECIFICATIONS

Catalog Number

645701

Size

25 microg

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Clone

DO-1

Antigen

P53 N-terminal linear epitope aa 20-25

Gene Id

7157

Isotype

Mouse IgG2a

Clonality

Monoclonal

Dilutions

Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 - 1 microg per ml antibody for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Tumor protein p53 (TP53)

Regulatory Status

RUO