Purified anti-p38 MAPK

p38 mitogen-activated protein kinase (MAPK) is a member of the stress activated kinase family with a molecular weight of 38 kD. MAPK can be activated by a number of stimuli including heat, changes in osmolarity, irradiation, and cold. The MAPKs require dual phosphorylation (threonine and tyrosine) for enzymatic activation. The p38 MAP kinase is required for G2 cell cycle delay after UV irradiation, also participates in cytokine production (IL-6 and erythropoietin). The Poly6224 antibody recognizes human, mouse, and rat p38 MAPK and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

622402

Size

200 microl

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Clone

Poly6224

Antigen

peptide-KLH, C terminal

Gene Id

80824

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store at -20 C.

Alternative Names

Stress activated kinase p38, p38 MAP kinase, stress activated protein kinase 2A

Regulatory Status

RUO