Purified anti-NPM/B23 Phospho (Thr234/Thr237)

NPM/B23 (also known as numatrin, nucleophosmin 1, nucleolar phosphoprotein B23) is a member of the nucleophosmin/nucleoplasmin family that shuttles between the nucleus and cytoplasm. This protein regulates the stability and transcriptional activity of p53 and acts as a molecular chaperone. NPM/B23 preferentially binds to denatured proteins and has been shown to stimulate DNA polymerase activity and control the duplication of centrosomes. The chaperone activity of NPM/B23 is regulated by protein kinase CK2 and promotes release of denatured substrates from NPM/B23. NPM/B23 is modified by phosphorylation onThr199. This protein has been shown to bind to RNA and DNA and interact with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens. The Poly6191 antibody recognizes human phosphorylated NPM/B23 (Thr234/Thr237) and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

619102

Size

200 microl

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6191

Antigen

Modified peptide

Gene Id

4896

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Numatrin, nucleophosmin 1, nucleolar phosphoprotein B23

Regulatory Status

RUO