Purified anti-NF-kappaB p65

NF-kappaB/p65 (nuclear factor kappa light chain enhancer in B cells p65) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-kappaB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-kappaB/p65 is bound (with p50) to IkappaB inhibitor in cytoplasm in inactive form. Phosphorylation of IkappaB releases and NF-kappaB which goes to the nucleus to activate gene expression. NF-kappaB can be activated with LPS, TNF-alpha, phorbol ester, or IL-1. The Poly6226 antibody reacts with the C-terminal region of human, mouse, and rat NF-kappaB p65 and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

622601

Size

50 microl

Applications

IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Clone

Poly6226

Antigen

peptide-KLH, C-terminal

Gene Id

4790

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

NFkappaB p65, nuclear factor kappa light chain enhancer in B cells p65

Regulatory Status

RUO