Purified anti-Neurofilament heavy protein (NF-H)

NF-H is an abundant, stable cytoplasmic protein located in neuronal cells in large axons frequently used as a cell type marker. The NF-H protein shares a high degree of structural and sequence homology with the NF-L and NF-M subunits, especially in the coiled-coil core domain. NF-M and NF-H form flexible extensions linking the neurofilament proteins to each other and other cytoplasmic proteins. Deletions and insertions in the NF-H protein have been reported in amyotrophic lateral sclerosis. The NF-01 monoclonal antibody reacts with a conserved phosphorylated epitope of NF-H present in all species and has been reported to be useful for Western blotting, immunohistochemistry using formalin-fixed, paraffin-embedded tissues and immunofluorescence staining.

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SPECIFICATIONS

Catalog Number

626402

Size

100 microg

Applications

ICC, IHC, WB

Hosts

Mouse

Clone

NF-01

Antigen

Pellet of pig brain cold stable proteins after microtubule depolymerization

Gene Id

380684

Isotype

Mouse IgG1

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 10 microg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

Neurofilament protein, heavy polypeptide; neurofilament heavy polypeptide

Regulatory Status

RUO