Purified anti-mouse Ki-67

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

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SPECIFICATIONS

Catalog Number

652402

Size

100 microg

Applications

FC, ICC, IF, IHC, WB

Hosts

Rat

Reactivities

Mouse

Clone

16A8

Antigen

E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.

Gene Id

17345

Isotype

Rat IgG2a, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microl per 5 ml antibody dilution buffer for each mini-gel (0.5microg/ml). Additionally, each lot of this antibody is quality control tested by our Ki-67 staining protocol below. Immunofluorescence microscopy, suggested working dilution(s): Use 5 microl per 1 ml antibody dilution buffer (2.5 microg/ml). It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

KIA, proliferation-related Ki-67 antigen, Ki67

Regulatory Status

RUO