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Purified anti-mouse Ki-67

Purified anti-mouse Ki-67

Cat no: 652401

Supplier: BioLegend
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The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.
Catalogue number: 652401
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 25 microg
Clone: 16A8
Gene: 17345
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microl per 5 ml antibody dilution buffer for each mini-gel (0.5microg/ml). Additionally, each lot of this antibody is quality control tested by our Ki-67 staining protocol below. Immunofluorescence microscopy, suggested working dilution(s): Use 5 microl per 1 ml antibody dilution buffer (2.5 microg/ml). It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store undiluted at 4C.
Concentration: 0.5 mg/ml
Antigen: E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Isotype: Rat IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: KIA, proliferation-related Ki-67 antigen
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