Purified anti-mouse IL-33

IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-kappaB and MAPK pathways via identical signalling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.

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SPECIFICATIONS

Catalog Number

516501

Size

100 microg

Hosts

Goat

Reactivities

Mouse, Rat

Clone

Poly5165

Antigen

Mouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli.

Isotype

Goat Ig

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.5-2 microg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000-15.6 pg/ml are recommended.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. Do not freeze.

Regulatory Status

RUO