Purified anti-mouse IL-1alpha

IL-1 refers to two proteins, IL-1alpha and IL-1beta which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1alpha is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The purified BL1a-72 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated BL1a-89 antibody as the detection antibody and recombinant mouse IL-1alpha?(Cat. No. 562801) as the standard.

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SPECIFICATIONS

Catalog Number

512402

Size

500 microg

Applications

IP, WB

Hosts

Rat

Reactivities

Mouse

Clone

BL1a-72

Antigen

E. coli - expressed, recombinant mouse IL-1alpha

Gene Id

16175

Isotype

Rat IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1alpha recombinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For Western Blotting applications, it is suggested to use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Interleukin-1alpha, IL-1F1, LAF, EP, LEM, MCF/MNCF

Regulatory Status

RUO