Purified anti-mouse IL-10

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-gamma, TNF-beta, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-alpha synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.

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SPECIFICATIONS

Catalog Number

505002

Size

500 microg

Applications

IHC

Hosts

Rat

Reactivities

Mouse

Clone

JES5-16E3

Antigen

E. coli-expressed, recombinant mouse IL-10

Gene Id

16153

Isotype

Rat IgG2b, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.1-1.0 microg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For ELISA capture, Carbonate buffer, pH 9.5 is highly recommended for coating buffer.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Interleukin-10 (IL10), CSIF, B-TCGF, BTCGF, TGIF

Regulatory Status

RUO