Purified anti-MCM2

The MCM2 protein (minichromosome maintenance protein 2) is a 120 kD member of the MCM family that forms a heterotetramer with other family members. This nuclear protein functions in eukaryotic genome replication. MCM2 forms a complex with MCM4, MCM6, and MCM7. MCM2 regulates the helicase activity of the pre-replication complex and is involved in the formation of replication forks. MCM2 is regulated by protein kinases Cdc2 and Cdc7 and can be modified by phosphorylation. The Poly6022 antibody recognizes the C-terminal region of both human and mouse MCM2 and has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

602201

Size

50 microl

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Clone

Poly6022

Antigen

Recombinant (partial), C-terminal

Gene Id

4171

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Minichromosome maintenance protein 2, Cdc19

Regulatory Status

RUO