Purified anti-human Pro-Major Basic Protein 1 (ProMBP1)

Pro-form of Major Basic Protein 1 (ProMBP1) is composed of a strongly acidic 90-residue propart and a highly basic mature portion of 117 residues. It is an extensively and very heterogeneously glycosylated proteoglycan. ProMBP1 is processed to 14 kD MBP1 by convertase during maturation and localized in cell granule core. The mature MBP1 is a cytotoxic protein. The mAb J175-7D4 binds ProMBP1 and intermediate ProMBP1, but not mature MBP1. It specifically stain basophils, does not stain eosinophils, granulocytes, and mast cells and can be used as a marker for human basophils.

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SPECIFICATIONS

Catalog Number

346802

Size

100 microg

Applications

FC, ICC, IHC, IP

Hosts

Mouse

Reactivities

Hum

Clone

J175-7D4

Antigen

Recombinant pro-form of human major basic protein 1

Gene Id

4153

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.5 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

ProMBP1

Regulatory Status

RUO