Purified anti-human IL-10

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production.

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SPECIFICATIONS

Catalog Number

501401

Size

50 microg

Applications

IHC, WB

Hosts

Rat

Reactivities

Hum

Clone

JES3-9D7

Antigen

COS - expressed, recombinant human IL-10

Gene Id

3586

Isotype

Rat IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Interleukin-10, B-TCGF, CSIF, TGIF

Regulatory Status

RUO