Purified anti-human CD340 (erbB2/HER-2)

The 24D2 recognizes human CD340 also known as HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, and oncogene NGL, neuroblastoma- or glioblastoma-derived. CD340 is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain with an approximate molecular weight of 185 kD. CD340 Contains three furin repeats and two cheY homologous receiver domains in the extracellular region. The HER2/erbB2 protein is expressed on many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, gliblastoma, some c-ALL blasts and bone marrow MSC. Although the erbB2/HER2 protein contains no ligand binding domain, this protein interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Over-expression of erbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers. The erbB2/HER-2 protein has been shown to interact with a large number of proteins including SHC1, EGF receptorm, neuroregulin 1, c-Src, integrin beta4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and beta-catenin, among others. CD340 is extensively modified by tyrosine phosphoryation on multiple residues (Y 1023, Y1139, Y1196, Y1221, Y1222, Y1248). The 24D2 antibody has been shown to be useful for flow cytometry.

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SPECIFICATIONS

Catalog Number

324402

Size

100 microg

Applications

FC, IF

Hosts

Mouse

Reactivities

Hum

Clone

24D2

Antigen

NIH-3T3 transfected with human HER-2

Gene Id

2064

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.125 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

erbB2, erbB-2, HER-2, HER2

Regulatory Status

RUO