Purified anti-human CD172a/b (SIRPalpha/beta)

CD172a, also known as signal-regulatory protein alpha (SIRPalpha), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily and is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion, and phagocytosis. CD47 (IAP) is the extracellular ligand for CD172a. SIRPalpha was recently demonstrated to be a specifc marker for cardiomyocytes derived from human pluripotent stem cells.2

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SPECIFICATIONS

Catalog Number

323801

Size

25 microg

Applications

FC

Hosts

Mouse

Reactivities

Hum

Clone

SE5A5

Antigen

NIH-3T3/hu-SIRPalpha cell line

Gene Id

140885

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?2.0 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

SIRPa, BIT, SHPS1, MFR, P84, PTPNS1

Regulatory Status

RUO