Purified anti-human CD148

CD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leucocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leucocytes. CD148 as a leucocyte activation marker and may be involved in the regulation of T cell activation. Leucocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors.

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SPECIFICATIONS

Catalog Number

328702

Size

100 microg

Applications

FC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Clone

A3

Antigen

PHA-stimulated human PBMC

Gene Id

5796

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ? 1.0 microg per 106 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

HPTP-eta, DEP-1, p260

Regulatory Status

RUO