Purified anti-Histone H3 Phospho (Ser28)

H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-alpha (mixed linage kinase-like mitogen activated protein triple kinase alpha) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.

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SPECIFICATIONS

Catalog Number

641002

Size

100 microg

Applications

FC, ICC, IF, IP, WB

Hosts

Rat

Reactivities

Hum

Clone

HTA28

Antigen

Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of Human Histone H3.

Gene Id

10919

Isotype

Rat IgG2a, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store at 4C.

Alternative Names

Histone-H3

Regulatory Status

RUO