Purified anti-H2A.X Phospho (Ser139)

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.

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SPECIFICATIONS

Catalog Number

613402

Size

100 microg

Applications

FC, ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Clone

2F3

Antigen

Modified peptide

Gene Id

3014

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this H2A.X antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 microg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store at 4C.

Alternative Names

H2A.x, H2a/x, Histone 2A, Histone 2A.X

Regulatory Status

RUO